In this lesson, students complete the steps required to clone a single gene and express it in a model organism.

• Students will explore how scientists are able to isolate and study single genes.
• Students will be introduced to recombinant DNA and the molecular biology of cloning.
• Students will become familiar with the process of bacterial transformation, as well as with important molecular biology tools, including restriction enzymes, plasmids and ligase.

• cloning
• palindrome
• sticky ends
• recombinant DNA
• transformation
• restriction enzyme
• plasmid
• ligase
• model organism

Using simulations, students complete the steps required to clone a single gene and express it in a model organism. Assessments are embedded in the activity to engage discussion and gauge learning.
As a result, students will:

• Learn about the process of molecular genetics, including restriction enzymes, ligation, DNA transformation and the use of model organisms.
• Learn how scientists isolate a gene, recombine DNA and express that DNA in bacteria.