Education Technology

Recombinant DNA

Published on 07/10/2011

Activity Overview

In this lesson, students complete the steps required to clone a single gene and express it in a model organism.

Objectives

  • Students will explore how scientists are able to isolate and study single genes.
  • Students will be introduced to recombinant DNA and the molecular biology of cloning.
  • Students will become familiar with the process of bacterial transformation, as well as with important molecular biology tools, including restriction enzymes, plasmids and ligase.

Vocabulary

  • cloning
  • palindrome
  • sticky ends
  • recombinant DNA
  • transformation
  • restriction enzyme
  • plasmid
  • ligase
  • model organism

About the Lesson

Using simulations, students complete the steps required to clone a single gene and express it in a model organism. Assessments are embedded in the activity to engage discussion and gauge learning.
As a result, students will:

  • Learn about the process of molecular genetics, including restriction enzymes, ligation, DNA transformation and the use of model organisms.
  • Learn how scientists isolate a gene, recombine DNA and express that DNA in bacteria.