Education Technology

Science: Vernier - Enzyme Action: Testing Catalase Activity
by Vernier Software & Technology

Published on June 09, 2008

Overview

Students will use an O2 Gas Sensor to measure the amount of O2 produced when H2O2 is broken down by the enzyme catalase at different concentrations, pH values, and temperatures. They will also measure the enzyme's initial reaction rates.

Key Steps

  • Image

    Students use the O2 Gas Sensor during the release of oxygen when hydrogen peroxide reacts with an enzyme catalase. The reaction is repeated at various pH levels, temperatures, and concentrations.

  • Image

    To find the initial rate of the enzyme reaction, students find the slope of the line on the steepest part of the graph.