Activity Detail
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Vernier - Enzyme Action: Testing Catalase Activity
 


Activity Overview

Students will use an O2 Gas Sensor to measure the amount of O2 produced when H2O2 is broken down by the enzyme catalase at different concentrations, pH values, and temperatures. They will also measure the enzyme's initial reaction rates.

Before the Activity

Print attached .pdf for each student. You will find one .pdf for EasyData and one .pdf for DataMate.

During the Activity

Distribute the pages to your class.

Follow the Activity procedures:
  • Use 10 mL of 1.5 % hydrogen peroxide
  • Start the enzyme-catalyzed reaction by adding five drops of enzyme suspension
  • Measure the amount of oxygen released every 3 seconds for a period of three minutes
  • Plot a graph of the amount of Oxygen gas released versus Time
  • Calculate the rate of reaction
  • Repeat the experiment using 10 drops and 20 drops of enzyme suspension
  • Calculate the rate of reaction at different enzyme concentrations
  • Repeat the experiment at different temperatures and different pH (pH 4, pH 7, and pH 10) and calculate the reaction rates

    		After the Activity

    		Students will fill in the Student Data Collection and Analysis Sheet.

    Review student results:
  • As a class, discuss questions that appeared to be more challenging
  • Re-teach concepts as necessary

    		Other Downloads




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    view textbook alignment

    Subject Area:
    Science : Biology : Biochemistry

    Author:
    Vernier Software & Technology

    Level:
    9-12

    Activity Time:
    60 Minutes

    Device:
    TI-83 Plus Family, TI-84 Plus Family

    Apps:
    DataMate, EasyData™

    Software:
    TI Connect™

    Accessories:
    Sensor - O2 Gas, CBL™/CBL 2™, TI Connectivity Cable, Vernier EasyLink®, Unit-to-Unit link cables, Vernier LabPro®

    Other:

  • 250-mL Nalgene bottle
  • Enzyme suspension
  • Three dropper pipettes
  • Thermometer
  • Three test tubes and test tube rack
  • 400-mL beaker
  • Water
  • 10-mL graduated cylinder
  • Ice
  • Graphical analysis
  • 1.5 % and 3.0 % hydrogen peroxide
  • pH buffers


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